Integrated Precision Medicine Technologies
Research Center of Excellence

Position: Cancer
Affiliation: The Cyprus Institute of Neurology and Genetics

male cv

Dr. Kleitos Sokratous, PhD (Male), is a postdoctoral researcher in Bioinformatics Group at The Cyprus Institute of Neurology and Genetics. His research interests lie in the areas of biomolecular mass spectrometry and omics as well as in the evolving field of computational modelling using mass spectrometry results. Mass spectrometry has evolved into a powerful analytical tool that can provide unique functional and structural information about protein–protein interactions, conformation, assembly and topology of biomolecular assemblies. Elucidation of structural and functional information of these assemblies would push forward the research for the development of novel therapeutic approaches. He obtained his BSc in Chemistry from the University of Athens in 2005 and his MSc in Analytical and Pharmaceutical Science from Loughborough University, UK, in 2007. During his MSc studies, he took an industrial placement at 3M Health Care, Loughborough, where he performed a short research project under the supervision of Dr Helen Reid. In 2009, he joined the group of Dr. Neil Oldham at the University of Nottingham for his PhD studies in Chemistry, with particular focus on biomolecular mass spectrometry. His research project was focused on the investigation and quantification of the nature of ubiquitin-ubiquitin binding domain interactions by electrospray ionization mass spectrometry (ESI-MS). The project lead to the development and application of an ESI-MS based approach to probe affinity and selectivity of ubiquitin-binding. In addition, during his doctoral studies he has employed ion mobility–mass spectrometry (IM–MS) approaches and molecular dynamic simulations to investigate the effects of cation adducts upon the gas-phase conformational changes of small proteins. Furthermore, he has employed Fmoc-SPPS approach to synthesise a series of hexapeptides that mimic the C-terminal of ubiquitin, which could act as potential inhibitors of ubiquitin-ubiquitin binding domain interactionsthat involve the C-terminal region of ubiquitin. After the completion of his doctoral studies, he moved to Cyprus and took a postdoc position at the “The Cyprus Institute of Neurology and Genetics. This position was part of a project that aimed in the establishment of the first MS-based proteomics facility. During this time, he took a leading role in the project and he worked closely with the head of the department and his collaborators on the appropriate selection of instruments and establishment of new protocols. After completion of the facility, he was responsible for the smooth operation of the facility, which is equipped with two MS instruments, a Synapt G2Si and a Xevo TQD, as well as two LC systems, a nanoAcquity UPLC and an Acquity I-Class UPLC. He has been involvedin multiple projects that aimed in the identification of novel biomarkers of Breast and Prostate cancer (Research Foundation Projects, Proteomics and Genomics of Breast and Prostate cancers). He currently participates in a European consortium that works towards the understanding of SLE (Systemic Lupus Erythematosus) genetics, proteomics and its translation to clinic (IMI funded project, PRECISESADS). In addition, he is involved in the supervision of two research projects (an MSc and a PhD) and he also delivers two lectures for a postgraduate course that is taking place at the Cyprus School of Molecular Medicine; MM102 (lecture entitled “The Human Proteome”).

Bioinformatics Group

The Bioinformatics Group has been recently established at CING headed by the Bioinformatics ERA Chair. Its research is focusing on Computational Diagnostics and Therapeutics inside the fields of Systems Bioinformatics and Medical Informatics. The Group exploits network-based bioinformatics methodologies along the axis of translational research to develop tailor-made applications of computer aided diagnosis and risk estimation regarding diseases of interest at CING. Also, the Group utilizes any kind of biomedical data available (from omics to imaging and other clinical data) to reconstruct and analyze multi-layered networks towards integromics and personalized medicine. Among the major Group’s research interests are the following: Network Analysis and networked biomarker discovery, Machine learning for computer aided diagnosis, Modeling and Simulation of Complex Biological Networks, Multi source and multilayer analysis towards precision medicine, In silico drug discovery and drug repurposing, Post-omic analysis and knowledge extraction, e-Health, m-Health and Assistive technologies informatics.

Department of Neurogenetics

The Neurogenetics Department occupies dedicated spaces of 100m2 for offices and laboratories. Furthermore, the Neurogenetics Department has access to core molecular genetics laboratory space and equipment that includes a NextSeq500, an AB 3130XL automatic analyzer, an ABI 7900 Real time PCR machine and a fully equipped tissue culture room.

Electron Microscopy/Molecular Pathology

The laboratory facilities of the Electron Microscopy/Molecular Pathology include; Laser Capture Microdissection (LCM) (Veritas LCC1704), confocal microscope, ZEISS brightfield and fluorescent microscope, Automated DNA sequencer – (ABI PRISM 310), dHPLC DNA fragment analysis (Transgenomics), Fast Real-time PCR system (ABI 7900 HT), genetic analysers 3130XL x3, Beckman analyzers x3, PCR Thermal Cyclers, Centrifuges (some with temperature control), SDS-PAGE & Western blotting Equipment, Dedicated Tissue culture Facility (with laminar flow hood and incubator) and Chemical hoods. In addition, the department hosts a newly established proteomics facility which consists of liquid chromatography systems (Waters nanoAcquity UPLC with 2D and Waters Acquity I-Class), a triple Quad MS system (Waters Xavo TQD) and a high resolution ion mobility mass spectrometry system (Waters Synapt G2Si) as well as an isoelectric focusing (IEF) system (Agilent 3100 OFFGEL fractionator).

Clinical Facilities

Clinical facilities include, in addition to the Outpatient Department, and the Neurology Ward, a laboratory of Clinical Neurophysiology and the laboratory of Neuropathology. The Transgenic Mouse Facility (TMF) is a core facility of the CING and is located in the basement of the CING building. The 5 CING Neurology Clinics are: Neurology Clinic A is predominantly involved with the clinical and laboratory evaluation (muscle and nerve biopsies) of patients with an emphasis on neuromuscular disorders such as, myopathies, motor neuron disease and neuropathies. The Clinic also has an interest in multiple sclerosis, neurodegenerative and neurogenetic disorders. Since 2002, a Familial Amyloidosis neuropathy clinic takes place once a month where a multidisciplinary service is offered. Neurology Clinic B provides tertiary medical care to patients with neurological disorders and other neurogenetic and genetic illnesses (epilepsy, sleep disorders, movement disorders, Alzheimer's disease, and other neurodegenerative disorders as well as behavioral problems that result from brain disease) that require specialized diagnosis and treatment. Neurology Clinic C is a Neurovascular laboratory which offers services in the field of Neurosonology. Colour coded duplex-doppler studies of the extracranial carotid arteries, the extracranial vertebral arteries and the intracranial arteries (both the anterior and the posterior circulations) are performed on a regular service basis. Neurology Clinic D is the Electromyography (EMG) Laboratory which specializes in electrodiagnostic examinations that evaluate, monitor, diagnose, and exclude disorders of the peripheral nervous system. Unlike imaging techniques such as magnetic resonance imaging (MRI) or computed tomography (CT), electrophysiological measures from peripheral nerves and/or muscles bestow information about their function. As electrodiagnostic studies are an extension of the patient’s clinical history and physical examination, each test session is designed and interpreted in parallel with that information. Consequently, our team is able to provide high-level patient care and diagnostic support to referring physicians and patients. Neurology Clinic E provides tertiary medical care to patients with various neurological disorders, focusing on neuromuscular (myasthenia gravis, peripheral neuropathies and myopathies, motor neuron disease), neurogenetic and other degenerative, autoimmune and demyelinating disorders of the peripheral and central nervous system. The Clinic also participates in the provision of specialized electrodiagnostic services (electromyography and nerve conduction studies) in the EMG lab. Clinic E includes the Neuroscience Laboratory mainly involved in translational neurological research, as well as the CING Mouse Facility, which serves as national core faciltiy providing services to researchers from CING and other institutions.

The Cyprus Institute of Neurology and Genetics Team

Eleni Zampa

Eleni Zampa

Neurodegenerative Disorders

The Cyprus Institute of Neurology and Genetics

Savvas Papacostas

Savvas Papacostas

Neurodegenerative Disorders

The Cyprus Institute of Neurology and Genetics

Marios Pantziaris

Marios Pantziaris

Neurodegenerative Disorders

The Cyprus Institute of Neurology and Genetics

Yiolanda Christou

Yiolanda Christou

Neurodegenerative Disorders

The Cyprus Institute of Neurology and Genetics

Christiana Demetriou

Christiana Demetriou

Neurodegenerative Disorders

The Cyprus Institute of Neurology and Genetics

Eleni Leonidou

Eleni Leonidou

Neurodegenerative Disorders

The Cyprus Institute of Neurology and Genetics

Ioanna Kousiappa

Ioanna Kousiappa

Neurodegenerative Disorders

The Cyprus Institute of Neurology and Genetics

Kyriacos Kyriacou

Kyriacos Kyriacou

Cancer

The Cyprus Institute of Neurology and Genetics

Andreas Hadjisavvas

Andreas Hadjisavvas

Cancer

The Cyprus Institute of Neurology and Genetics

Kyriaki Michaelidou

Kyriaki Michaelidou

Cancer

The Cyprus Institute of Neurology and Genetics

Maria Loizidou

Maria Loizidou

Cancer

The Cyprus Institute of Neurology and Genetics

Kyproulla Christodoulou

Kyproulla Christodoulou

Molecular/ Cellular Biosciences (translational –Omics)

The Cyprus Institute of Neurology and Genetics

Paschalis Nicolaou

Paschalis Nicolaou

Molecular/ Cellular Biosciences (translational –Omics)

The Cyprus Institute of Neurology and Genetics

Christina Votsi

Christina Votsi

Molecular/ Cellular Biosciences (translational –Omics)

The Cyprus Institute of Neurology and Genetics

Antonis Ververis

Antonis Ververis

Molecular/ Cellular Biosciences (translational –Omics)

The Cyprus Institute of Neurology and Genetics

George Spyrou

George Spyrou

Bioinformatics & Systems Medicine

The Cyprus Institute of Neurology and Genetics

Anastasios Oulas

Anastasios Oulas

Bioinformatics & Systems Medicine

The Cyprus Institute of Neurology and Genetics

Margarita Zachariou

Margarita Zachariou

Bioinformatics & Systems Medicine

The Cyprus Institute of Neurology and Genetics

George Minadakis

George Minadakis

Bioinformatics & Systems Medicine

The Cyprus Institute of Neurology and Genetics

Kleitos Socratous

Kleitos Socratous

Cancer

The Cyprus Institute of Neurology and Genetics